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Separation and analysis of monomer and dimer core complexes. (A) Fractionation of detergent-solubilised LH2, core monomer and dimer complexes from wild-type and mutant strains on sucrose density gradients. 1—wild-type; 2—PufX−; 3—PufX R49L; 4—PufX R53L; 5—PufX R49/53L; 6—PufX G52L; 7—PufB Y43F; 8—PufB Y43L; 9—ΔPufBA. (B) Immunoblot of photosynthetic membrane samples corresponding to those in the upper panel, probed with antibodies to PufX.
