Fig. 5.

Myriocin blocks de novo SL synthesis without altering JB-induced cytotoxicity and cell vacuolation. HGC-27 cells were cultured with 5 μM myriocin (Myr) or ethanol for 2 h and then incubated with 5 μM JB for 16 h. A: SLs were analyzed by UPLC-TOF MS and HPLC MS/MS. *P < 0.05, ***P < 0.0005 (n = 3). B: HGC-27 cells were treated with 5 μM of Myr (or ethanol) for 2 h and then incubated with different concentrations of JB for 16 h. Cell viability was determined by MTT. Results are the mean ± SD of two experiments in triplicate. C: Phase contrast images are representative of the phenotype observed in two independent experiments in triplicate. Scale bar: 50 μm.