Fig. 3.

Sequence and predicted structures of an IRE-like stem-loop in Cyp7a1 mRNA. A: Sequence encoding the canonical IRE RNA stem loops in the 3′-UTR of TFR1 aligned to Cyp7a1 IRE, AHSP IRE, and Dgkk IRE. The CAGUG terminal loop of the IRE is shown in red letters. B: RNA secondary structure of the Cyp7a1 IRE-like, TFR1 IREb, AHSP, and Dgkk IRE was assessed by the software. C: Quantitative RT-PCR of hepatic Cyp7a1 and Dgkk mRNA levels in AFLD mice and control mice. D: Western blot analyses of CYP7A1 protein levels in AFLD mice and control mice. GAPDH blots represent tissue protein loading controls. #P > 0.05, *P < 0.05.