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. 2017 May 23;58(8):1548–1560. doi: 10.1194/jlr.M074534

Fig. 4.

Fig. 4.

Iron regulates the intracellular and hepatic Cyp7a1 levels. A: DFO increased Cyp7a1 and IRP2 mRNA in a dose-dependent manner. Five independent experiments for each time point. B: Western blots of CYP7A1, IRP2, and Ft-L were performed. C: Cyp7a1 and IRP2 mRNA were detected by quantitative RT-PCR. D: Western blots of CYP7A1, IRP2, and Ft-L were performed. E: Hepa1-6 cells were treated with alcohol for the indicated concentrations. DFO was added after alcohol treated for 48 h, cells were harvested after 24 h, and Cyp7a1 mRNA levels were measured. F: Iron removal affects the expression of CYP7A1 in vivo. The expression of CYP7A1, IRP2, and Ft-L were measured, and hepatic iron content was measured. G: Iron loading affects the expression of CYP7A1 in vivo. The expression of CYP7A1, IRP2, and Ft-L were measured. #P > 0.05, *P < 0.05, **P < 0.01 (n = 3).