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Homologous competition assays were employed. Cellular extracts were incubated with either 50 pmol biotinylated TFR1 IREb or Cyp7a1 IRE in the presence of a 0, 5, 10 or 25-fold TFR1 IRE (A) or in the presence of a 0, 10, 25 or 50-fold excess of nonbiotinylated Cyp7a1 IRE (B).
