Fig. 5.

APCI mass spectra of 18:1(n-9)/16:0-Chl in the negative ion mode. Full scan spectrum (A). CID MS² spectrum of [M − H − Chl + H₂O]⁻ (m/z 535.5; normalized collision energy 15%) (B). The chloroform solution (1 mg/ml) delivered by a syringe pump (5 μl/min) was mixed with acetonitrile:ethyl acetate (45:55, by volume) flowing at 150 μl/min. The mixture was directly infused into the ion source.