Colonization with pNAE-EcN increases NAE levels in extra-intestinal tissue of Nape-pld−/− mice. Total tissue NAE levels 2 weeks post-administration were measured by LC/MS/MS. A: Omental adipose tissue (one-way ANOVA: P < 0.0001; Tukey’s multiple comparisons test: pNAPE-EcN vs. pEcN, P < 0.01; pNAE-EcN vs. pEcN, P < 0.001; pNAE-EcN vs. pNAPE-EcN, P < 0.01). B: Retroperitoneal adipose tissue (one-way ANOVA: P = 0.0283; Tukey’s multiple comparisons test: pNAPE-EcN vs. pEcN, P = not significant (n.s.); pNAE-EcN vs. pEcN, P < 0.05). C: Brain (one-way ANOVA: P = 0.0741). D: Liver (one-way ANOVA: P = 0.8519).