Fig. 3.

Formation of JA-Ile conjugates after 3 weeks under different conditions and two time curves. A: Different growth conditions in CDB and a comparison with PDB. Light reduced biosynthesis and it was augmented in shaking flasks (100 rpm). Changing from CDB to PDB and increasing the temperature to 28°C yielded the largest amounts of jasmonates and this growth condition was used routinely. B: Partial time curves for biosynthesis of (−)-JA-Ile and (+)-JA-Ile by two different cultures in PDB, which were inoculated from liquid cultures in PDB. Left: The biosynthesis of (+)-JA-Ile and (−)-JA-Ile reached 4.5 and 0.25 mg l⁻¹, respectively, after 8 days. Right: The biosynthesis of (+)-JA-Ile and (−)-JA-Ile reached over 0.22 and 0.01 g l⁻¹, respectively, after 15 days.