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. 2017 Jun 1;58(8):1670–1680. doi: 10.1194/jlr.M077305

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Fig. 6. — Oxidation of 18:3n-3 by mycelia of Fot during short-time incubation in 0.1 M NaBO₃ (pH 8.2). A: MS/MS analysis of products. Top: MS/MS analysis of HOTrE (m/z 293). Bottom: MS/MS analysis of KOTrE (m/z 291). The insets mark the elution of the identified products, 16- and 17-HOTrE, and 16-KOTrE, respectively. Small amounts of 12-OPDA (marked by arrow) eluted 3 min before 16-KOTrE. B: Chiral HPLC-MS/MS analysis (m/z 293 → full scan) of 16-HOTrE [m/z 235 (A⁻-58); top] and separation of 17-HOTrE [m/z 249 (A⁻-44); bottom].