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. 2017 Jun 20;58(8):1692–1701. doi: 10.1194/jlr.M077479

Fig. 3.

Fig. 3.

Adaptive changes in hepatic and intestinal gene expression in LD-fed mice of the indicated genotypes. A, B: RNA was extracted from liver or scraped proximal intestinal mucosa, and mRNA abundance was determined by Real-time quantitative PCR. A: Hepatic mRNA expression. B: Proximal intestinal mRNA expression. C: NPC1L1 and SR-B1 protein expression in proximal intestinal mucosa. Thirty micrograms of protein was separated by SDS-PAGE gel, and the indicated proteins were detected by Western blot (upper). Band density was quantitated, and the data are presented as the mean ± SE (lower). D: Protein was extracted from terminal ileum and aliquots of total homogenate (Fgf15) or purified apical membranes (Asbt) separated on SDS-PAGE gel, and the indicated protein was detected by Western blot. The relative abundance of Fgf15 and Asbt were quantitated by using a Kodak image system, and the data are presented as the mean ± SE. *Statistically significant differences (P < 0.05).