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. 2017 Jun 20;58(8):1692–1701. doi: 10.1194/jlr.M077479

Fig. 6.

Fig. 6.

Gallbladder contractility is increased in Cd36−/− mice. A: Mice of the indicated genotype were euthanized after a 4 h fast, and the gallbladder was visualized after laparotomy performed under anesthesia. B: Cd36 mRNA expression in gallbladder showed loss of Cd36 expression in Cd36−/−mice. C: No change in Cd36 mRNA expression comparing WT, Cd36 flox, Cd36-IKO, and Cd36-LKO gallbladder. D, E: Gallbladder contractility was studied in vitro in response to the indicated agonists. Increased contractility was found in gallbladders from Cd36−/− mice in response to both potassium chloride (D) and methacholine (E), compared with congenic WT controls. F: The contractile force of gallbladders of the indicated genotype is shown. G: RNA was extracted from gallbladders of the indicated genotype of LD-fed mice, and mRNA expression of candidate genes was determined by qPCR. The data are the mean ± SE. *Statistically significant differences (P < 0.05). H: Morphological appearance of gallbladders from LD-fed mice of the indicated genotype (hematoxylin and eosin stain; magnification ×200). I: Gallbladder lipids were extracted and quantitated enzymatically (Materials and Methods). Gallbladder lipid content is expressed as μg/mg protein. The data in B–G and I represent the mean ± SE, n = 3–6 per group. CE, cholesteryl ester; FC, free cholesterol; TC, total cholesterol; TG, triglyceride.