Figure EV4. FMRP binds to G‐rich sequence in MMP‐9 3′UTR .

1. Sequence used to prepare the rat MMP‐9 RNA probe that was used in the RNA electrophoretic gel‐shift assay shown in (B).
2. The labeled MMP‐9 RNA probe was incubated in the absence (lane 1) or presence of increasing amounts of purified FMRP (70–860 nM, lanes 2–5). 20× and 200× molar excess of unlabeled probe was added as competitor to the reactions with 860 nM FMRP to confirm the specificity of the interaction (lanes 6–7, respectively). The figure shows a representative image from three independent experiments.