Figure EV4. Replication (SA promoter)‐dependent GFP transgene is selectively expressed in EpCAM ⁺ tumour cells.

• A, B
  Total cells from a peritoneal ascites sample were incubated with EnAd‐CMV‐GFP or EnAd‐SA‐GFP, with uninfected cells serving as a negative control. After 3 days, wells were imaged by bright‐field or fluorescence microscopy. Original magnification ×10; scale bar, 100 μm (A). Total cells were harvested, and the proportion of GFP⁺ cells that were CD3‐, CD11b‐ or EpCAM‐positive determined using flow cytometry (B). Each condition was measured in biological quadruplicate and represented as mean ± SD. Significance was assessed by comparison to uninfected control wells using a one‐way ANOVA test with Tukey's post hoc analysis, *P < 0.05, **P < 0.01, ***P < 0.001.

Source data are available online for this figure.