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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Virology. 2017 Jun 9;509:52–59. doi: 10.1016/j.virol.2017.06.004

Figure 3.

Figure 3

A) HERV-K108 Env expression inhibits HIV-1 virion production in cell culture supernatant. HIV-1 virion release in the tissue culture supernatant was measured 42h after transfection by ELISA for capsid p24. The results are represented as p24 production relative (%) to control, which was obtained by HIV-1 co-transfection with empty control vector. The results shown reflect the averages of three independent experiments and error bars represent standard deviations. B) HERV-K108 Env expression only minimally affects HIV-1 infectivity. TZM-bl reporter cell line was infected with 2.2ng of CA-p24 equivalents of HIV-1 produced in the presence of increasing amounts of HERV-K 108 env plasmid. Β-galactosidase was measured 44 hours after infection. The results presented are the averages of three independent experiments and error bars represent standard deviations. C) HERV-K108 Env expression in HeLa cells inhibits HIV-1 prduction. Western blot of lysates of HeLa cells transfected with increasing amounts of HERV K108 env alongside plasmids encoding HIV-1 LAI. Quantifications represent the average of 3 experiments and the error bars represent the standard deviation from the mean.