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. 2017 Jun 1;9(8):1150–1164. doi: 10.15252/emmm.201707604

Figure 1. Cardiac αKOγKO mice exhibit FTT with impaired liver GH signaling.

Figure 1

  • A
    Daily body weight of αKOγKO and littermate control mice. n = 55–145 mice per group with both genders included.
  • B
    Representative picture of 10‐day‐old αKOγKO and littermate control αHetγWT mice.
  • C, D
    Plasma GH and IGF1 concentrations in 10‐ (C, n = 7–10 mice per group) and 16‐day‐old mice (D, n = 9–11 mice per group) measured by ELISA.
  • E
    Phosphorylated (Tyr694) and total STAT5 in 10‐day‐old mouse livers determined by Western blot (n = 3–4 mice per group). β‐Actin serves as a loading control.
  • F
    Relative levels of phosphorylated and total STAT5 and JAK2 in 13‐day‐old mouse livers (normalized to total protein content of individual mouse liver) were quantified by ELISA (n = 13 mice per group).
  • G
    Expression of STAT5 target genes Igf1, Igfbp3, and Igfals in 10‐day‐old mouse livers measured by qPCR (n = 7–8 mice per group).
  • H
    Liver (normalized to total protein content, n = 15–16 mice per group) and plasma (n = 9–10 mice per group) IGFBP3 concentrations in 13‐day‐old mice measured by ELISA.
Data information: *P < 0.05, **P < 0.01, ****P < 0.0001, *****P < 0.00001, and ******P < 0.000001 between αKOγKO and all other control genotypes by t‐test. All values are mean + s.e.m. Source data are available online for this figure.