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. 2017 Jun 1;31(11):1089–1094. doi: 10.1101/gad.302067.117

Figure 1.

Figure 1.

BUB-1 promotes APC/C activation and is required to recruit a dynamic CDC-20 pool to the kinetochore. (A) Images from a time-lapse sequence of the indicated GFP fusions in embryos also coexpressing mCherry(mCh)::H2b. Bar, 5 µm. (B) Metaphase images (top) and quantification (bottom) of GFP::CDC-20 chromosomal fluorescence for the indicated conditions. Error bars are the 95% confidence interval. (****) P < 0.0001 (Mann-Whitney test). Bar, 2 µm. (C) FRAP analysis. (Top) Metaphase images of GFP::CDC-20-expressing or KNL-1::GFP-expressing embryos. Time 0 indicates the first bleached frame. (Bottom) Quantification of GFP fluorescence. Error bars are the 95% confidence interval. Bar, 1 µm. (D) Images from time-lapse sequences (left) and quantification of the nuclear envelope breakdown (NEBD)–anaphase onset interval (right) for the indicated conditions. Time below panels is in seconds relative to NEBD. P-values are from Mann-Whitney test. Bar, 5 µm. (E) Quantification of the NEBD–anaphase onset interval for the indicated conditions. Imaging was performed at 22°C, at which the Apc8MAT-3 temperature-sensitive mutant is viable. (****) P < 0.0001 (Mann-Whitney test). (F) Plot of normalized Cyclin B::mNeongreen (mNG) fluorescence for the indicated conditions. Error bars are the 95% confidence interval; anaphase onset times are indicated. See also Supplemental Figure S1.