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. Author manuscript; available in PMC: 2017 Dec 5.
Published in final edited form as: Nat Neurosci. 2017 Jun 5;20(7):934–942. doi: 10.1038/nn.4574

Figure 6. Inactivation of CGRPPBN neurons attenuates sickness behaviors in LLC cancer model.

Figure 6

a-b, Hourly locomotor activity of mice with intact (GFP, n = 8) or inactivated (TetTox, n = 7) CGRPPBN neurons, prior to tumor implantation and 13 d after implantation (Pre-LLC, two-way repeated measures ANOVA: Interaction, F(22, 286) = 1.12, p = 0.3222; Time, F(22, 286) = 39.31, p < 0.0001; LLC, two-way repeated measures ANOVA: Interaction, F(1, 286) = 4.48, p < 0.0001; Time, F(22, 286) = 15.91, p < 0.0001; Treatment, F(1, 286) = 14.73, p = 0.0021). Grey-shaded region on × axis represents dark-cycle. c-d, Total distance traveled and average velocity of locomotor activity during 23-h recording period of GFP and TetTox mice prior to and after tumor implantation (Total Distance, two-way repeated measures ANOVA: Interaction, F(1, 13) = 6.21, p = 0.0270; Time, F(1, 13) = 15.91, p = 0.0096; Treatment, F(1, 13) = 4.76, p = 0.0480; Avg Velocity, two-way repeated measures ANOVA: Interaction, F(1, 13) = 2.95, p = 0.1099; Time, F(1, 13) = 4.69, p = 0.0495; Treatment, F(1, 13) = 8.05, p = 0.0140). e-g, Measurements from open-field anxiety test of GFP and TetTox mice prior to and 15 d after tumor implantation (Time in Center, two-way repeated measures ANOVA: Interaction, F(1, 13) = 7.19, p = 0.0189; Time, F(1, 13) = 2.40, p = 0.1453; Treatment, F(1, 13) = 5.64, p = 0.0337; Distance Moved, two-way repeated measures ANOVA: Interaction, F(1, 13) = 1.99, p = 0.1814; Time, F(1, 13) = 26.04, p = 0.0002; Treatment, F(1, 13) = 0.92, p = 0.3547; Time in Hunched Posture, two-way repeated measures ANOVA: Interaction, F(1, 13) = 20.23, p = 0.0006; Time, F(1, 13) = 16.40, p = 0.0014; Treatment, F(1, 13) = 15.35, p = 0.0018). h-j, Measurements from elevated-plus maze anxiety test of GFP and TetTox mice prior to and 15 d after tumor implantation (Time in Open Arm, two-way repeated measures ANOVA: Interaction, F(1, 13) = 14.60, p = 0.0021; Time, F(1, 13) = 16.74, p = 0.0013; Treatment, F(1, 13) = 1.032, p = 0.3283; Distance Moved, two-way repeated measures ANOVA: Interaction, F(1, 13) = 1.59, p = 0.2299; Time, F(1, 13) = 1.02, p = 0.3310; Treatment, F(1, 13) = 0.28, p = 0.6045; Time in Hunched Posture, two-way repeated measures ANOVA: Interaction, F(1, 13) = 9.82, p = 0.0079; Time, F(1, 13) = 5.92, p = 0.0302; Treatment, F(1, 13) = 16.26, p = 0.0014). k-l, Quantification and representative images (24-h time point) of nesting behavior before and 16 d after tumor implantation (two-way ANOVA: Interaction, F(15, 156) = 3.01, p = 0.0003; Time, F(5, 156) = 228.40, p < 0.0001; Treatment, F(3, 156) = 44.61, p < 0.001). m, Daily food intake of GFP and TetTox mice after tumor implantation (two-tailed student's t-test on day 17: t(13) = 6.36, p < 0.0001). TetTox mice were pair-fed to the intake of GFP mice until day 16, after which TetTox mice were allowed ad libitum access to food. Line graphs show mean ± SEM. Box plots show mean (+), median, quartiles (boxes) and range (whiskers) for each treatment. * P ˂ 0.05, ** P ˂ 0.01, *** P ˂ 0.001. See also Supplementary Fig. 8.