Table S1.
Primer | Sequence |
---|---|
Forward ARMS2_LOC1 | 5′-CAATGGTAGCCAGGACCCAT-3′ |
Reverse ARMS2_LOC2 | 5′-ATCCGTTAAGTCGGAAGGAG-3′ |
Forward CD14_F | 5′-TTGGTGCCAACAGATGAGGTTCAC-3′ |
Reverse CD14_R | 5′-TTCTTTCCTACACAGCGGCACCC-3′ |
Forward TLR4_1 | 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′ |
Reverse TLR4_2 | 5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′ |
Forward TLR4_3 | 5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3′ |
Reverse TLR4_4 | 5′-CCTGAAGACTGGAGAGTGAGTTAAATGCT-3′ |
Note: The underlined bases in both forward primers of TLR4 gene (TLR4_1 and TLR4_3) denote an altered base that was introduced in order to create either an NcoI (Asp299Gly) or a HinfI (Thr399Ile) restriction site.
Abbreviations: PCR-RFLP, polymerase chain reaction – restriction fragment length polymorphism; SNP, single-nucleotide polymorphism.