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. 2017 Jul 26;11:1347–1358. doi: 10.2147/OPTH.S134538

Table S1.

Sequences of primers used in PCR-RFLP genotyping methods of SNPs in ARMS2, CD14, and TLR4 genes

Primer Sequence
Forward ARMS2_LOC1 5′-CAATGGTAGCCAGGACCCAT-3′
Reverse ARMS2_LOC2 5′-ATCCGTTAAGTCGGAAGGAG-3′
Forward CD14_F 5′-TTGGTGCCAACAGATGAGGTTCAC-3′
Reverse CD14_R 5′-TTCTTTCCTACACAGCGGCACCC-3′
Forward TLR4_1 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′
Reverse TLR4_2 5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′
Forward TLR4_3 5′-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3′
Reverse TLR4_4 5′-CCTGAAGACTGGAGAGTGAGTTAAATGCT-3′

Note: The underlined bases in both forward primers of TLR4 gene (TLR4_1 and TLR4_3) denote an altered base that was introduced in order to create either an NcoI (Asp299Gly) or a HinfI (Thr399Ile) restriction site.

Abbreviations: PCR-RFLP, polymerase chain reaction – restriction fragment length polymorphism; SNP, single-nucleotide polymorphism.