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. 2017 Jul 26;10:3743–3754. doi: 10.2147/OTT.S142610

Figure 3.

Figure 3

Knockdown of KIAA1522 expression reduces the tumorigenicity of esophageal carcinoma cells in nude mice.(p)(p)Notes: KYSE510 and KYSE150 cells were infected with lentiviruses expressing KIAA1522 shRNA (shKIAA) or negative control shRNA (shCtrl), and stable cell clones were selected with puromycin. (A) Western blot analysis of KIAA1522 expression levels in stable cell clones. The arrow indicates the position of KIAA1522 specific band. Relative ratios of absorbance for KIAA1522 to GAPDH were plotted. The data are presented as the mean ± SEM. ***P<0.001. (B) Colony formation assay. (C–E) KYSE150-shKIAA or shCtrl stable cell clones were implanted subcutaneously into nude mice (n=10 per group), the tumor volumes were measured every 3 days for 3 weeks, and sc tumors were excised 21 days after inoculation. (C) Tumor growth curves. The data are presented as the mean ± SEM. **P<0.01, ***P<0.001. (D) Tumor weight. ***P<0.001; (E) Photographs of sc tumors. Scale bar =1 cm.(p)(p)Abbreviations: Ctrl, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; sc, subcutaneous; sh, shRNA.