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. 2017 Aug 1;12(8):e0181868. doi: 10.1371/journal.pone.0181868

Fig 3. RORγt or RORα siRNA effect on IL-17A transcript in human CD4 T cells during Th17 differentiation.

Fig 3

Human CD4 T cells purified from 2 donors were activated under Th17 differentiation condition as described in Material and Methods. RORγt and RORα siRNAs, as well as control siRNAs were transfected into CD4 T cells prior to Th17 differentiation and samples were collected at different time points as indicated. Expression of RORγt (A), RORα (B) and IL-17A (C) transcripts was measured by RT-PCR. IL-17A cytokine released in culture supernatants (D) was measured by ELISA. Statistical significance of the difference between RORC or RORA siRNA treated samples compared to mock samples at different time points was analyzed with 2-way ANOVA and P values were presented as *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001.