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. 2017 Aug 1;12(8):e0181868. doi: 10.1371/journal.pone.0181868

Fig 4. RORγt inverse agonist effect on IL-17A transcript in human CD4 T cells during Th17 differentiation.

Fig 4

Human CD4 T cells purified from 2 donors were activated under Th17 differentiation condition as described in Material and Methods. CD4 T cells were treated with three RORγt inverse agonists at two different concentrations as well as DMSO vehicle control. Expression of IL-17A mRNA in different treated samples was measured by RT-PCR at time points as indicated (A). IL-17A cytokine released in culture supernatants (B) was measured by ELISA. Statistical significance of the difference between compound treated to DMSO samples at different time points was analyzed with 2-way ANOVA and P values were indicated as *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001.