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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Nutr Food Res. 2017 Jan 3;61(4):10.1002/mnfr.201600746. doi: 10.1002/mnfr.201600746

Fig. 4.

Fig. 4

Resveratrol enhanced the expression of brown adipogenic marker genes in differentiated iBAT SVCs after 7-day brown adipogenic differentiation. A) Relative mRNA expression of PRDM16, UCP-1, Cidea and PGC-1a in differentiated iBAT SVCs of wild-type. B) UCP1 immunofluorescence staining of differentiated iBAT SVCs. Nuclei were stained with DAPI (scale bar, 100 µm). C) Western blot analysis of PRDM16UCP1, PDH and Cyto C of differentiated iBAT SVCs. β-actin was used as loading control. D) Mean ± SEM of immunoblotting bands and the intensities of the bands were expressed as arbitrary units. *P < 0.05, **P < 0.01 and *** P < 0.001 versus control.