Fig 2. C-Abl expression in developing microfilariae decreases over the course of maturation.

C-Abl expression was measured as mean fluorescence intensity in the internal structures of adult worms. (A, C) The intensity of fluorescence between the reproductive apparatus (A, uterus, ovaries, early morula, pretzel; C, vas deferens, spermatids), muscle, glycogen, and intestine is significantly more in the structures treated with anti-c-Abl antibody compared with isotype control. (A) Adult females show over the course of embryonic development a decrease in c-Abl expression from the early morula to the pretzel stage (p = 0.0035). Higher c-Abl expression is also seen in muscle compared with glycogen in females (A, p = 0.0187) as well as males (C, p = 0.0155). (B, D) Fluorescence intensity generated by the nuclear stain DAPI, is essentially identical between the c-Abl and isotype control conditions. Three sections per condition were analyzed, and the experiment was repeated 3 times; means ± SEM.