Figure 4. Targeted deletion of the domain II β‐sheets renders ERdj3 a stable dimer.

1. Illustration showing the domain architecture for ^RERdj3^WT, ^RERdj3^ΔII, or ^RERdj3^QEVV.
2. Immunoblot of Superdex 200 gel filtration fractions of ^RERdj3^WT, ^RERdj3^ΔII, or ^RERdj3^QEVV is shown. The migration of molecular weight standards is shown above.
3. Analytical ultracentrifugation (AUC) sedimentation velocity c(S) distribution of ^RERdj3^ΔII (0.8 mg/ml). The inset shows the extrapolated ${\mathrm{s}}_{20,\mathrm{w}}^0$ value for ^RERdj3^ΔII and the predicted molecular weight calculated using a frictional coefficient of 1.5.
4. Analytical ultracentrifugation (AUC) sedimentation velocity c(S) distribution of ^RERdj3^QEVV (0.87 mg/ml). The inset shows the extrapolated ${\mathrm{s}}_{20,\mathrm{w}}^0$ value for ^RERdj3^QEVV and the predicted molecular weight calculated using a frictional coefficient of 1.5.

Source data are available online for this figure.