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. 2017 Jun 29;36(15):2233–2250. doi: 10.15252/embj.201696113

Figure EV1. Transcriptional response to inhibitors of OGT and OGA (related to Fig 1).

Figure EV1

  1. OGT and OGA inhibitors reduced and increased, respectively, global O‐GlcNAc levels. MDA‐MB‐231 cells were treated with 50 μM 5SGlcNAc or 25 μM Thiamet‐G for 24 h and WCLs were analyzed by Western blots.
  2. qPCR validation of indicated up‐ and downregulated genes from microarray data. MDA‐MB‐231 cells were treated with DMSO vehicle or 50 μM 5SGlcNAc for 48 h and extracted mRNA was analyzed by qPCR (normalized to β‐actin). n = 3, error bars represent standard deviation.
  3. Lack of correlation between low‐OGT activity (i.e., 5SGlcNAc) and PI3K or EGFR signaling gene expression signatures when projected into the Miller breast tumor dataset. r = Pearson's correlation coefficient.

Source data are available online for this figure.