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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Immunol. 2017 Jun 30;199(3):1003–1011. doi: 10.4049/jimmunol.1700256

Figure 3.

Figure 3

Rapid germinal center (GC) reactions LNs after ZIKV challenge. A) Representative flow cytometry data showing a sequential gating strategy to enrich for proliferating B cells: 1) Live cells; 2) Lymphocytes; 3–4) Singlets; 5) CD3−/CD20+ cells; and 6) Ki67+ cells at days 7 and 14 after ZIKV challenge. Proliferating B cells (CD3−/CD20+/Ki67+) and formation of B cell follicles presented increased frequency in the LNs at day 14 by flow cytometry (A) and tissue immunofluorescence (B). C) Representative flow cytometry data showing a Tfh cell gating. During formation of B cell follicles, increased frequency of CD4 and CD8 Tfh cells (CD3+/CD20−/CD8−/+/PD1hi/CXCR5+/CCR7−) were detected in the LNs at day 14 of challenge by flow cytometry (C) and tissue immunofluorescence assays (D).