Figure 4.

Evolutionary relationships between the assembly lines of the newly identified cyclic lipopeptides and structurally related molecules, based on their constituent phylogenetic groups of adenylation domains, as indicated in Supplementary Figure 12. Each NRPS module is represented by its A-domain, along with its substrate specificity. Colored lines connect A-domains in adjacently depicted assembly lines that belong to the same phylogenetic group; note that some clades contain multiple substrates due to recent evolution of the substrate specificity of an individual member (e.g., Val-10 in the orfamide BGC), and that some substrate specificities are split across two clades (e.g., Glu-2 of the WLIP BGC originates from a different Glu-specific clade),. Color gradient represents a cosine similarity score between two given A-domains.