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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Neuron. 2016 Dec 15;93(1):33–47. doi: 10.1016/j.neuron.2016.11.045

Figure 4. Synaptic specificity of anterograde viral spread.

Figure 4

(A) TdTomato expression (red) and GFAP labeling (green) in the corpus callosum (upper) and pontine nucleus (lower) following injection of AAV1-CMV-Cre into V1 of an Ai14 mouse. High-magnification images (right panels) show that Nissl stained cell bodies (blue) within the corpus callosum were negative for tdTomato (upper), and that tdTomato-positive cell bodies within PN were negative for GFAP staining (lower). Scale bars: 250 µm, left panels; 25 µm, right panels.

(B) Slice recording from transneuronally labeled neurons in the striatum (red) following co-injection of AAV1-hSyn-Cre and AAV1-EF1a-DIO-ChR2-YFP into V1 (left panel). Top right image shows ChR2-expressing axons (green) surrounding tdTomato-labeled striatal neurons. Scale: 25 µm. Middle panel, average LED-evoked excitatory (−70 mV) and inhibitory (0 mV) currents in an example tdTomato+ striatal neuron before and after perfusing in TTX and 4AP. LED stimulation is marked by a blue bar. Right bottom, a summary of amplitudes of average monosynaptic excitatory currents evoked by LED in 9 recorded striatal cells.