Figure 3. High titers of autologous and heterologous neutralization achieved in guinea pigs.
(A) Immunization scheme for guinea pigs. Week 18 sera were used for neutralization assays.
(B) Neutralization of wild type and 4-glycan-deleted viruses by week 18 sera of guinea pigs immunized with fully glycosylated and glycan-deleted immunogens. Immunogenicity is expressed as log10(ID50) with respect to autologous and heterologous strains for each immunogen. The standard error for individual animals is displayed following the “±” sign. WT, wild type virus; ΔGly4, 4-glycan-deleted virus.
(C) Targeted glycan-removal increases sensitivity of 4-glycan-deleted viruses to antibodies targeting the CD4 supersite.