Figure 11. An ERK inhibitor promoted AMPAR rectification in fasted but not fed mice.
(a) I-V relationship of AMPAR-EPSCs in POMC neurons in fed mice at 0 (blue), 10 (box), and 15 (red) min after application (in vitro) of ERK inhibitor, UD98059 (10 µM in recording pipette solution). The value of peak amplitude of AMPAR-mediated eEPSC was normalized as percent by the value at −60 mV. The dotted line represents a linear regression of AMPAR-EPSCs in POMC neurons between −80 and 0 mV at 0 min after application of UD98059. (b) I-V relationship of AMPAR-EPSCs in POMC neurons in fasted mice at 0 (blue), 10 (box), and 15 (red) min after application of UD98059. (c) RIs of AMPAR-EPSCs in POMC neurons in fed (blue) and fasted (red) mice treated with UD98059. (d, e) I-V relationship of AMPAR-EPSCs and (f) RI in POMC neurons excluded poor space clamp recordings did not change from original results. (mean ± SEM. * and ** indicates p<0.05 and 0.01, respectively, paired t-test.).