Fig. 4.

PC-TP-interacting protein Them2 does not promote hepatic injury in response to the MCD diet. A: hepatic Them2 mRNA expression in WT and Pctp^−/− mice fed a MCD diet for 5 wk. WT and Them2^−/− mice were fed a chow or the MCD diet for 5 wk. B: representative images of hematoxylin and eosin-stained liver sections (×40 magnification; white bar = 50 µm). NAFLD activity score (NAS; C) and NAS components (D). Plasma activities of alanine aminotransferase (ALT; E) and aspartate aminotransferase (AST; F). G: relative abundance of macrovesicular and microvesicular lipid droplets in livers of MCD diet-fed mice. Plasma concentration of β-hydroxybutyrate (H) and hepatic malondialdehyde (MDA; I). Values represent means ± SE. A: n = 7–15. C–I: n = 3–7. *P < 0.05, Pctp^−/− vs. WT; §P < 0.05, MCD vs. chow diet.