Table 1.
Oligonucleotides used for qPCR
| Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| Phosphatidylcholine transfer protein; Pctp | CCAGAGTATCTCGGCACCTC | ACGCTTTCACCATGTCCTC |
| Sterol regulatory element binding protein-1c; Srebp1c | TGGATTGCACATTTGAAGACAT | GGCCAGAGAAGCAGAAGAGA |
| Carbohydrate response element binding protein-alpha; Chrebpa | CGACACTCACCCACCTCTTC | TTGTTCAGCCGGATCTTGTC |
| Acetyl-CoA carboxylase; Acaca | GAGAGGGCTCAAGTCCTTCC | ACATCCACTTCCACACACGA |
| Fatty acid synthase; Fasn | TGGGTTCTAGCCAGCAGAGT | ACCACCAGAGACCGTTATGC |
| Diacylglycerol acyltransferase 3; Dgat1 | GGCCCAAGGTAGAAGAGGAC | GATCAGCATCACCACACACC |
| Diacylglycerol acyltransferase 4; Dgat2 | AGGATCTGCCCTGTCACG | GCCAGCCAGGTGAAGTAG |
| Glycerol-3-phosphate acyltransferase 3; Gpat3 | TACATGCCTCCCATGACTAG | ATCCGTTGCCCACGATCATC |
| Glycerol-3-phosphate acyltransferase 4; Gpat4 | TGTCTGGTTTGAGCGTTCTG | TTCTGGGAAGATGAGGATGG |
| Fibroblast growth factor 21; Fgf21 | CTGGGGGGTCTACCAAGCATA | CACCCAGGATTTGAATGACC |
| Monocyte chemoattractant protein-1; Mcp1 | GTTGGCTCAGCCAGATGCA | AGCCTACTCATTGGGATCATCTTG |
| Tumor necrosis factor-alpha; Tnfa | CACAAGATGCTGGGACAGTGA | TCCTTGATGGTGGTGCATGA |
| Matrix metalloproteinase-9; Mmp9 | TGTCTGGAGATTCGACTTGAAGTC | TGAGTTCCAGGGCACACCA |
| Transforming growth factor-beta; Tgfb | GTGTGGAGCAACATGTGGAACTCTA | TTGGTTCAGCCACTGCCGTA |
| Alpha-smooth muscle actin; Acta2 | TCCTGACGCTGAAGTATCCGATA | GGTGCCAGATCTTTTCCATGTC |
| c-jun | GCCAACCTCAGCTTCAA | CCTGAGACTCCATGTCGATAG |
| CCAAT/enhancer-binding protein; Chop | GGGCCATAGAACTCTGACTGGA | CATCCCAAAGCCCTCGCTCTCCA |
| Inositol-requiring enzyme 1-alpha; Ire1a | CCCAAATGTGATCCGCTACT | TTGAGAGAATGCAGGTGTGC |
| Spliced X-box-binding protein-1; Xbp1s | GAGTCCGCAGCAGGTG | GTGTCAGAGTCCATGGGA |
| Unspliced X-box-binding protein-1; Xbp1u | AAGAAACACGCTTGGGAATGG | ACTCCCCTTGGCCTCCAC |
| Activating transcription factor 6; Atf6 | GCCCAGACTGTTTTGCTCTC | CCCATACTTCTGGTGGCACT |
| Liver receptor homolog-1; Lrh1 | CCCTGCTGGACTACACGGTTT | GCTAATGGGAGATGTGACAAA |
| Polo-like kinase 3; Plk3 | ACCTACAGCACCGCCATATC | CGCAGGTAGTAGCGAACCTC |
| Sterol 12-α-hydroxylase; Cyp8b1 | GCCTTCAAGTATGATCGGTTCCT | GATCTTCTTGCCCGACTTGTAGA |
| Small heterodimer partner 1; Shp1 | AACCTGCCGTCCTTCTGCCA | AACCTGCCGTCCTTCTGCCA |
| Activating transcription factor 2; Atf2 | CTCCAGCTCACACAACTCCA | TGTTTCAGCTGTGCCACTTC |
| Thioesterase superfamily member 2; Them2 | TCGACCATGGCTCTAATGTG | TCCTGTGGGTCTTGTTGGTCA |
| Ribosomal protein L32; Rpl32 | CACCAGTCAGACCGATATG | TTCTCCGCACCCTGTTG |