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The β-catenin antibody recognizes β-catenin in immunoprecipitation (IP) samples from nuclear fraction of rat inner medullary collecting duct (IMCD) cells. “Input” is pre-IP sample; “IP” is bound material eluted from protein G beads after IP using β-catenin antibody or preimmune IgG as control. An equal amount of IgG was used for preimmune and β-catenin IPs. “Unbound” is material that did not attach to the protein G beads. Loading was on an equal-volume basis (15 µl per lane).
