Fig. 2.

Effect of PT-GS-KO on phosphate-dependent glutaminase (PDG), phosphoenolpyruvate carboxykinase (PEPCK), Rhbg, Na⁺-H⁺ exchanger, isoform 3 (NHE3), and Na⁺-K⁺- 2Cl⁻ transporter, isoform 2 (NKCC2) in dietary protein restriction. Immunoblot analyses of renal cortical and medullary protein homogenates from Control and PT-GS-KO mice fed 6% protein diet for 7 days are shown. There were no significant differences between Control and PT-GS-KO mice in expression of multiple proteins involved in renal ammonia handling, including PDG, PEPCK, Rhbg, NHE3, and NKCC2. Immunoblot data were not normally distributed for the following proteins: PDG in the outer stripe of the outer medulla (OSOM), PEPCK in cortex and OSOM, Rhbg in cortex and OSOM, and NKCC2. Nonparametric analysis was used to compare median expression levels. NS, not significant. Student’s t-test was used for the remaining analyses. Values are means ± SE; n = 6 mice/genotype.