Fig. 4.

KRT20 expression in bladder tissues from mice with SCI. Bladders from sham-treated mice (A) or those with SCI (B–E) were fixed and processed for confocal microscopy. Tissues were immunolabeled with a KRT20-specific antibody, TRITC-phalloidin, and TO-PRO-3. A representative region of urothelium in which superficial umbrella cells (UC) bordered regions containing small superficial cells (SC) was imaged, and a projection of a Z-stack is shown for each condition. The sham and SCI experiments included 3 animals per group and were performed on 5 separate occasions. Representative images are presented.