Table 1.
Antibodies used in this study
| Target | Cell Type Labeled | Concentration Used | Species and Ab Type | Vendor/Source (Catalog No.) | 2° Ab or Blocking Ab | Vendor (Catalog No.) |
|---|---|---|---|---|---|---|
| BrdU | Proliferating cells | 1:200 | Mouse mAb | EMD Millipore (NA61) | Alexa 488-Goat anti-mouse | ThermoFisher Scientific (A-11001) |
| KRT5 | Intermediate and basal cells | 1:1,000 | Rabbit pAb | BioLegend (905501) | Dylight 549-Goat anti-rabbit | Jackson Immuno Research (111–505–144) |
| KRT14 | Sub population of basal cells | 1:300 | Chicken pAb | BioLegend (906001) | TRITC-Donkey anti-chicken | Jackson Immuno Research (703–025–155) |
| KRT20 | Umbrella cells | 1:1,000 | Rabbit pAb | Abcam (ab53120) | Alexa 488-Goat anti-rabbit | Jackson Immuno Research (111–545–144) |
| MKI67 (alias Ki-67) | Proliferating cells | 1:100 | Rabbit pAb | Abcam (15580) | Alexa 488-Goat anti-rabbit | Jackson Immuno Research (111–545–144) |
| TP63 | Intermediate and basal cells | 1:100 | Goat pAb | R&D Systems (AF1916) | Dylight 650-Donkey anti-goat | Abcam (ab96938) |
| UPK3a | Umbrella cells and KRT5− intermediate cells | 1:5 | Mouse mAb supernatant | Apodaca laboratory (see Ref. 55) | Alexa 488-Goat anti-mouse Goat-anti mouse Fab | Jackson Immuno Research (115–545–166) (115–007–003) |
| Pan-UPK (anti-AUM) | Umbrella cells and KRT5− intermediate cells | 1:1,000 | Rabbit pAb | T.T. Sun, NY University | Alexa 488-Goat anti-rabbit | Jackson Immuno Research (111–545–144) |