Fig. 6.

Exaggerated albuminuria in Podo-Atg7-KO mice during adriamycin treatment. A: body weight, serum urea nitrogen, and creatinine. n = 5; *P < 0.05 compared with the adriamycin; Podo-Atg7-WT group. B: urine samples were collected for SDS-PAGE followed by Coomassie blue staining. Albumin was loaded to show the main protein in urine samples was albumin. The representative gel staining shows proteinuria is mainly albuminuria. The representative gel shows that adriamycin induced significantly higher albuminuria in Podo-Atg7-KO mice. C: urinary albumin excretion ratio (UACR). The UACR was determined in Podo-Atg7-WT and Podo-Atg7-KO mice treated with adriamycin (19.5 mg/kg) or saline as control. n = 5; *P < 0.05 vs. adriamycin; Podo-Atg7-WT group. The results indicate that adriamycin induced significantly higher urinary albumin excretion in Podo-Atg7-KO mice than Podo-Atg7-WT mice.