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. 2017 Apr 6;313(1):L92–L103. doi: 10.1152/ajplung.00323.2016

Fig. 1.

Fig. 1.

Stable transfection of let-7d (antifibrotic) and miR-154 in B-MSCs is successful. Human bone-mesenchymal stem cells (B-MSCs) were stably transfected with let-7d, miR-154, or an empty vector control using lentiviral particles. To confirm successful transduction, we evaluated the presence of green fluorescent protein (GFP) using a fluorescent microscope, due to the expression of GFP in the expression lentivirus construct. Using FACS, we quantified the efficiency of the transduction (A). We also evaluated let-7d and miR-154 expression levels using qPCR (n = 3) (B and C). *P < 0.05.