Fig. 4.

LINC00341 guides polycomb repressive complex 2 (PRC2) to the VCAM1 promoter. A, B: HUVECs were transfected with siLINC00341 (siL341) or control siRNA (Ctrl) and treated with atorvastatin for 24 h. The EZH2 and its associated complex were immunoprecipitated from nuclear lysates with anti-EZH2 antibody. The amount of LINC00341 RNA (A) and VCAM1 promoter DNA (B) in PRC2 immunoprecipitates was determined by RT-qPCR. C: HUVECs were transfected with the VCAM1 promoter construct in the presence or absence of siL341 and treated with TNF-α and atorvastatin as indicated. Promoter activity was then assessed by luciferase assays. RLU, relative luminescence units. D: schematic illustration of the mechanism by which LINC00341 regulates the endothelial inflammatory status.