(A–B) Magnetic Resonance Imaging (MRI) of KPC
orthotopic tumors n=9 mice per group (A) and each
individual tumor (B). (C) Tumor volume as measured
by MRI n=9 mice per group. One-way ANOVA. (D)
Representative axial images. (E) Tumor weight (g: grams) at the
experimental end point. siScrbl iExo: n=9 mice,
siKrasG12D iExo: n=8 mice. (F) Change in
the percentage of mouse body weights, pre and post treatment (endpoint),
n=9 mice per group. (G) Representative gross images of
two KPC orthotopic mice that died on day 16 PTS (siScrbl iExo) or was
euthanized on day 16 PTS (siKrasG12D iExo). (H)
KrasG12D transcript levels in KPC689 cells
(n=3 independent experiments). One-way ANOVA. (I)
Kaplan-Meier curve of KPC orthotopic tumor bearing mice, Log-rank Mantel-Cox
test. siScrbl iExo group: n=9 mice, siKrasG12D iExo
group: n=8 mice. (J) Macroscopic metastatic nodules,
n=9 mice per group. (K) H&E stained tissues. Unless
stated otherwise, the data is presented as the mean ± SEM and
unpaired two-tailed t test was used to determine statistical significance.
* p<0.05, ** p< 0.01,
*** p< 0.001,
**** p<0.0001. See accompanying source
data.