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. Author manuscript; available in PMC: 2018 Oct 10.
Published in final edited form as: J Control Release. 2016 Dec 24;263:139–150. doi: 10.1016/j.jconrel.2016.12.025

Fig. 5.

Fig. 5

Effect of molecular weight on apoptosis induction of Raji B-cells. Percentage of apoptotic cells were analyzed by Annexin V/PI binding and quantified by flow cytometry. Incubation time was 24 h. Non-treated, cells in culture medium; Prem, premixture of Fab′1F5-PEG2-MORF1 (1 μM) and conjugates with different valences (P2-(PEG2-MORF2)12.7 or P3-(PEG2-MORF2)13.7 or P3-(PEG2-MORF2)25.1; 1 μM, MORF2-eqv). Cons, consecutive, Fab′1F5-PEG2-MORF1 (1 μM) followed (1 h later) by conjugates with different valences (P2-(PEG2-MORF2)12.7 or P3-(PEG2-MORF2)13.7 or P3-(PEG2-MORF2)25.1; 1 μM, MORF2-eqv). Percentage of apoptotic cells was quantified by flow cytometry. Statistics, unless otherwise indicated, was performed by comparing each consecutive treatment with premixed treatment (***p < 0.0001, **p < 0.005, *p < 0.05, n.s.: no significant difference). All data are presented as mean ± SD (n = 3).