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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2017 Feb 1;173:157–167. doi: 10.1016/j.jsbmb.2017.01.024

Figure 2. RIPK1 did not phosphorylate VDR in in vitro immunocomplex kinase assays.

Figure 2

A. His-tagged VDR proteins were expressed in bacteria, purified using nickel beads, and stained with Coomassie blue. B. Flag-RIPK1 was transfected into 293T cells and immunoprecipitated with anti-Flag antibodies. In vitro immunocomplex kinase assays were performed with purified His-VDR protein as a substrate. Myelin basic protein (MBP) was included as a positive control.