Figure 3.

Gene transfer pattern by FuG-E vector via retrograde gene delivery in the monkey brain. (A) Schematic diagram of the projections that innervate the striatum (STR) in macaque monkeys. CM-PF, centromedian -parafascicular complex of the thalamus; SNc, substantia nigra pars compacta. (B) Coordinates for intrastriatal injections. Representative injection sites in the caudate nucleus (Cd) and putamen (Put) are shown in a magnetic resonance image. (C,D) Immunohistochemical detection of transgene expression. The FuG-E–pseudotyped vector encoding GFP transgene (7.0 × 10¹⁰ genome copies/ml, 3–5 μl/ site × 16 sites) was injected into the Cd and Put in a macaque monkey and brain sections through the STR (C) and other brain regions (D) were immunostained with anti-GFP antibody. A number of immunopositive cells were detected in various neuronal populations in cortical areas including the primary motor cortex (M1), thalamic (CM), and nigral (SNc) regions innervating the STR. Right images are magnified views of the squares in the left image. LV, lateral ventricle. Scale bar: 2 mm.