Table 3.
Concentrations of products and salt inhibiting by 50% (IC50) the activity of the four putative P5C reductases from B. subtilis.
| ProH | ProI | ProG | ComER | |
|---|---|---|---|---|
| Proline (NADPHastheco−substrate) [mM] | 41.4 ± 4.2 | 145 ± 19 | 22.3 ± 2.1 | >1,000 |
| Proline (NADHastheco−substrate) [mM] | 667 ± 112 | 232 ± 43 | 175 ± 17 | >1,000 |
| NAD+ (NADPHastheco−substrate) [mM] | >50 | 23.6 ± 4.1 | >50 | 24.8 ± 4.5 |
| NAD+ (NADHastheco−substrate) [mM] | 44.5 ± 11.6 | 6.22 ± 0.60 | >50 | 29.7 ± 3.7 |
| NADP+ (NADPHastheco−substrate) [mM] | 19.0 ± 7.7 | 4.80 ± 0.62 | 4.39 ± 1.11 | not inhibitory |
| NADP+ (NADHastheco−substrate) [mM] | 0.66 ± 0.10 | 0.34 ± 0.04 | 0.23 ± 0.03 | 0.48 ± 0.07 |
| NaCl (NADPHastheco−substrate) [mM] | 484 ± 43 | 367 ± 41 | 745 ± 191 | not inhibitory |
| NaCl (NADHastheco−substrate) [mM] | 462 ± 75 | 208 ± 38 | 416 ± 78 | 114 |