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. 2017 Aug 2;8:1273. doi: 10.3389/fpls.2017.01273

FIGURE 1.

FIGURE 1

Genotyping of BAK1 (Y610F)-Flag lines in bak1-4 bak1-4/bkk1-1 bkk1-1 background. (A,B) Genomic organization of the T-DNA insertion knockout lines, bak1-4 (SALK_116202) and bkk1-1 (SALK_057955), both in the Col-0 ecotype. The position of each T-DNA insertion is depicted by an inverted triangle, and arrows showing positions of primers used for genotyping. (C) PCR using BAK1 gene specific primers (upper gel panel) and a T-DNA specific primer (lower gel panel). (D) PCR using BKK1 gene specific primers (upper gel panel) and a T-DNA specific primer (lower gel panel). The three lines used for genotyping are independent lines; WT is wild-type Col-0 that served as a positive control for gene specific primers and negative control for T-DNA specific primers.