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. 2017 Aug 2;8:1273. doi: 10.3389/fpls.2017.01273

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Copyright © 2017 Singh, Perraki, Kim, Shrivastava, Lee, Zhao, Schwessinger, Oh, Marshall-Colon, Zipfel and Huber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Similar growth and BL responses in transgenic plants expressing BAK1-Flag or BAK1 (Y610F)-Flag in the double null background. (A) Phenotype of 18- and 45-day-old Col-0, BAK1-Flag and BAK1 (Y610F)-Flag plants (in the bak1-4 bak1-4/bkk1-1 bkk1-1 double null background) grown in a normal long day photoperiod. (B) Immunoblot analysis indicates similar expression levels of the BAK1-Flag transgene in the different genotypes. Seedlings were grown in liquid MS medium for 10 days. Protein was immunoprecipitated using immobilized anti-Flag antibodies of solubilized microsomal membranes, and immunoblotting was performed with anti-Flag antibodies. The reactive band at 50 kDa (asterisk) is the heavy chain of the immobilized M2 antibody released from the Anti-Flag M2 Beads by elution with SDS-PAGE sample buffer containing reductant. (C) Dose-dependent hypocotyl elongation of 7 days old seedlings of BAK1-Flag, BAK1 (Y610F)-Flag and det2 at 0, 10, 100, and 1000 nM BL. Seedlings were grown in the long day photoperiod.