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. 2017 Aug 2;8:1338. doi: 10.3389/fpls.2017.01338

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Copyright © 2017 Fang, Bai, Huang, Wang, Wang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OsNPF7.3 is located in the rice vacuolar membrane. (A–C) Free GFP expression in rice protoplasts. (D–F) OsNPF7.3-GFP expression in rice protoplasts. (G–I) Fluorescence of OsNPF7.3-GFP coexpressed with the vacuolar membrane marker AtTPK-mkate in transiently transformed rice protoplasts. (J–L) Fluorescence of OsNPF7.3-GFP expressed in tobacco epidermal cells. Presented here are fluorescence images of GFP (A,D,G,J), corresponding bright-field images (B,E,K), AtTPK-mkate fluorescence images (H) and merged images (C,F,I,L). Bars = 5 μm for (A–L).