FIGURE 2.

PopP2^Pe_1 and PopP2^Pe_2 are avirulent in Arabidopsis, PopP2^Pe_13 is not. (A) PopP2 natural variants, PopP2^Pe_1 and PopP2^Pe_2, elicit an HR in resistant Arabidopsis accession Ws-2. The truncated natural variant, PopP2^Pe_13 is not recognized. PopP2 variants were delivered by Pseudomonas syringae pv. tomato (Pto) DC3000 and photographs were taken 1 day post-infiltration (1 dpi). Red asterisks indicate HR. This experiment was conducted three times with similar results. (B) Agrobacterium-mediated co-expression of PopP2 variants with RPS4 and RRS1-R in tobacco leaf cells. Red asterisks indicate strong programmed cell death (PCD) at 3 dpi. This experiment was conducted three times with similar results. (C) The PopP2 natural variant proteins accumulate to a similar amount in Nicotiana benthamiana after agro-infiltration. Immuno-detection of PopP2 variants C-terminally fused to YFP epitope tag was conducted using anti-GFP antibodies. Ponceau S staining of total protein demonstrates equal loading of the samples.