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. 2017 Aug 2;8:507. doi: 10.3389/fphar.2017.00507

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Copyright © 2017 Milton, Lacerda, Sinoti, Mesquita, Prakasan, Coelho, de Lima, Martini, Pazzine, Borin, Amato and Neves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dibutyltins promote adipogenesis in 3T3-L1. Cells were induced to differentiate with insulin, and exposed to vehicle (DMSO), Rosi 0.1 μM, TBTC 0.1 μM, DBTA 0.1 μM, DBTC 0.1 μM, DBTDL 1 μM, or DBTM 0.1 μM. (A) After 14 days the cells were fixed, stained with oil red O, and photo documented. (B) Western blot to evaluate the expression of FABP4 protein after 14 days of differentiation. (C) Real-time quantitative PCR to evaluate the expression of Fabp4, Adipoq, and Glut4 genes after 3 days of differentiation. Data are presented as mean (SD) of three independent experiments conducted in triplicate and expressed as activation relative to transcript levels in vehicle samples (DMSO). ^∗p ≤ 0.05.