FIGURE 3.

Dibutyltins’s adipogenic effect is PPARγ dependent. (A) 3T3-L1 cells were induced to differentiate with insulin, and exposed to vehicle (DMSO), Rosi 0.1 μM, TBTC 0.1 μM, DBTA 0.1 μM, DBTC 0.1 μM, DBTDL 1μM, or DBTM 0.1 μM in the presence or absence of a PPARγ specific antagonist, T0070907 1 μM. After 14 days of differentiation, the cells were fixed and stained with oil red O, and photo documented. (B) Cells were differentiated for 3 days and collected for gene expression evaluation of Fabp4 by real-time quantitative PCR. Data are presented as mean (SD) of three independent experiments conducted in triplicate and expressed as activation relative to transcript levels in vehicle samples (DMSO). ^∗p ≤ 0.01 (compared to vehicle in the absence of T0070907), and ^#p ≤ 0.001 (compared to the same ligand in the presence and the absence of T0070907).